| Abstract Detail
Ecological Section Annis, Jenna [1], O'Brien, Jennifer [2], Coons, Janice [3], Coutant, Nancy [2]. Breaking Seed Dormancy of Penstemon tubiflorus. Penstemon tubiflorus, commonly known as white wand beardtongue, is a prairie forb of conservation concern in Illinois. This study investigated four seed treatments to break seed dormancy: hickory smoke seasoning solution, prairie plant ash solution, stratification, and light vs. dark. For the hickory smoke, the following concentrations were used: 1:10, 1:100, and 1:1000. The prairie plant ash solution was created by burning tallgrass prairie species and the ash was used to create the solutions at the following concentrations: 2 g/L, 4 g/L, 8 g/L, and 16 g/L. Seeds were moist stratified for 1 or 2 months at 4oC. For the light vs. dark study, dishes with seeds for the dark treatment were wrapped with aluminum foil. For each treatment, plastic petri dishes were lined with 3 sheets of filter paper and moistened with 5 mL of appropriate solution. Distilled water was used for the control. All petri dishes were placed in a plastic tub and stored in a Percival Scientific seed germination chamber with constant light at 38 ± 5 µmol·m-2·s-1 at 25oC ± 0.1 for 3 weeks. Three replications were used for each treatment with 50 seeds per dish. Germinated seedlings were counted. Hickory smoke seasoning increased germination at concentrations below 1:10, compared to the control. Prairie plant ash solution at 8 g/L resulted in highest seed germination, compared to other ash treatments. Both 1 and 2 months of stratification increased germination significantly, compared to the control. Light combined with most successful hickory smoke concentration (1:100) showed a significant increase in germination compared to the dark treatments. Overall, the hickory smoke solution at 1:100 with light resulted in the highest germination percentage of all tested treatments. Broader Impacts:
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1 - Eastern Illinois University, Biological Sciences Department, 600 Lincoln Avenue, Charleston, IL, 61920, USA
2 - Eastern Illinois University, Biological Sciences, 600 Lincoln Ave, Charleston, IL, 61920, USA
3 - Eastern Illinois University, 600 Lincoln Ave, CHARLESTON, IL, 61920-3099, USA
Keywords:
none specified
Presentation Type: Poster:Posters for Sections
Session: P
Location: Battelle South/Convention Center
Date: Monday, July 9th, 2012
Time: 5:45 PM
Number: PEC010
Abstract ID:295 |
