| Abstract Detail
Ecological Section Remya, Chellappan [1], Bradley, Roach [5], Kanimozhi, Kasinathan [2], Panneerselvam, Annamalai [3], Thajuddin, Nooruddin [4], Gunasekaran, M [5]. Bio Conversion of Agro Industrial Waste for the Production of Amylase by the Fungus Rhizopus microsporus. Amylase is the starch hydrolyzing enzyme and one among the most important enzyme in present day biotechnology. In the present study an attempt was made to identify suitable agro industrial waste for amylase production in solid state culture. Rhizopus microsporus was isolated from coir retting soil. R.microsporus was cultured with various agro industrial waste substrates such as banana peel, tapioca peel, cabbage leaf, and wheat bran for amylase production. Among them, tapioca peel produced high amount of amylase (69.58U/g substrate). Optimum production of amylase at 60% moisture and maximum production was at pH 7.0. For the supplementary of carbon (cellobiose, glucose, lactose, maltose, starch and xylose) and nitrogen (ammonium chloride, ammonium sulphate and peptone) sources, xylase (96.77 U/g) have found to be the best source for maximum amylase production. Effect of various particle sizes study (0.1 mm, 2.0 mm and 4.0 mm), tapioca peel with the particle size of 4 mm produced high amylase activity. Thus this study revealed that tapioca peel is a key for production of amylase by R.microsporus in solid state fermentation. Broader Impacts:
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1 - Sri Pushpam Colle, Botany and Microbiology, Poondi, Tamilnadu, 613503, India
2 - Sri Pushpam Colle, , Botany and Microbiology, , Poondi, Tamilnadu, , 613503, India
3 - Sri Pushpam College, Botany and Microbiology, Poondi, Tamilnadu, 613503, India
4 - Bharathidasan University, Microbiology, Tiruchirappalli, Tamil Nadu, 320020, India
5 - Fisk University, Biology, 1000 17th Ave North, Nashville, TN, 37208, USA
Keywords:
Rhizopus microsporus
Agro industrial wastes.
Fermentation
amylase.
Presentation Type: Poster:Posters for Sections
Session: P
Location: Battelle South/Convention Center
Date: Monday, July 9th, 2012
Time: 5:45 PM
Number: PEC015
Abstract ID:344 |
