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Abstract Detail

Systematics Section/ASPT

Moore, Michael [1], Mandala, Venkata [1], Douglas, Norman [1], Brunner, Anna [2], Croley, Matthew [1], Stalberg, Gabriel [1], Ochoterena Booth, Helga [3], Flores Olvera, Hilda [3].

Seeing the forest and the trees: Reconstructing the phylogenetic and intraspecific history of Acleisanthes (Nyctaginaceae) using plastid genomics and targeted sequencing.

Acleisanthes A.Gray is a genus of ~16 species with a Chihuahuan Desert center of distribution. To improve resolution of deeper relationships in Acleisanthes, we generated complete plastid genome sequences for most taxa by multiplexing genomic DNAs on an Illumina GAIIx. To evaluate intraspecific variation within the genus, we also sequenced four chloroplast spacer regions and ITS for a broad population sampling of most taxa, including all widely distributed species. We place the results in the context of the assembly of arid- and semiarid-adapted floras in North America, including the assembly of the Chihuahuan Desert gypsum endemic flora, and we discuss the benefits of this dual approach for building a more complete picture of the evolutionary history of a clade.

Broader Impacts:

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Related Links:
Phylogenetics and phylogeography of Chihuahuan Desert gypsum endemics

1 - Oberlin College, Department of Biology, 119 Woodland St., Oberlin, OH, 44074, USA
2 - University of Michigan, School of Natural Resources and Environment, 440 Church St., Ann Arbor, MI, 48109, USA
3 - Universidad Nacional Autónoma de México, Instituto de Biología, Apartado Postal 70-233, México, DF, 04510, Mexico

next generation sequencing
plastid genomics
Illumina GAIIx
gypsum endemism
Chihuahuan Desert
intraspecific variation.

Presentation Type: Oral Paper:Papers for Sections
Session: 53
Location: Delaware C/Hyatt
Date: Wednesday, July 11th, 2012
Time: 1:45 PM
Number: 53002
Abstract ID:397

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