Ephedra pollination and other gnetalean oddities
Von Aderkas, Patrick , Little, Stefan , Pirone, Cary , Cross, Tyra , Hardie, Darryl , Smith, Derek , Han, Jun , Jesse, Susannah , Prior, Natalie , Thornburg, Robert , Bolinder, Kristina , Hou, Chen , Ickert-Bond, Stefanie M. , Borchers, Christoph , Rydin, Catarina .
Degradome and proteome of pollination drops of Ephedra and other gymnosperms (Gnetidae, Ginkgoidae and Pinidae).
We reveal the presence of a degradome in gymnosperm pollination drops. To study protein and peptide composition, we harvested pollination drops from species of Ephedra, Ginkgo, Taxus, and Chamaecyparis. The plants were growing outdoors or in greenhouses. Drops were pooled either by species or, in the case of more abundant samples,by collection date. Samples were processed by one of two methods: 1. gel electophoresis followed by processing of cored bands and capillary liquid chromatography; or by 2. liquid-liquid extraction and chromatographic separation. For peptidomics (masses < 10 kDa) samples were analyzed using a12T Bruker-Daltonics FT-ICR mass spectrometer or Thermo Scientific LTQ Orbitrap Velos. Larger masses (>10kDa) were injected into a Thermo Scientific LTQ Orbitrap Velos or an Applied Biosystems/Sciex QTRAP 4000.The proteome of Pinidae was richer than Ginkgoidae or Gnetidae. Degradomes of hundreds of peptide fragments were found in all species investigated, whether insect- or wind-pollinated. The presence of such a diversity of peptides in pollination drops presents opportunities to explore a different level of pollen-ovule interactions and ecological services.
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1 - University of Victoria, Centre for Forest Biology, Department of Biology, Victoria, BC, V8W 3N5, Canada
2 - University Of California, Davis, Mail Stop 1, One Shields Avenue, Davis, CA, 95616, USA
3 - Arnold Arboretum Of Harvard, Botany, 1300 Centre St, Roslindale, MA, 02131, USA
4 - UVic-Genome BC Proteomics Centre, University of Victoria, Victoria, BC, V8W 3N5, Canada
5 - Iowa State University, Dept of Biochemistry, Biophysics and Molecular Biology, Ames, IA, 50011, USA
6 - Stockholm University, Botany, Lilla Frescativägen 5, Stockholm, SE-106 91, Sweden
7 - UA Museum Of The North, Museum Of The North Herbarium, 907 Yukon Dr., Fairbanks, AK, 99775, USA
Presentation Type: Symposium or Colloquium Presentation
Location: Delaware A/Hyatt
Date: Monday, July 9th, 2012
Time: 8:00 AM