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Abstract Detail

Conservation Biology

Ko, Ya-Zhu  [1], He, Chin-Shang  [2], Chen, Chaur-Tzuhn  [3], Chiang, Yu-Chung [1].

Genetic diversity and conservation strategy of Amentotaxus formosana H. L. Li based on population genetics and MCMC assignment test.

In order to save the critically endangered species from extinction, to evaluate the genetic diversity, estimate the effective population size, and identify the distinct genetic units are the basic information to development the conservation strategy. Amentoaxus formosana H. L. Li. is an island endemic conifer restricted in the Hengchun Peninsula, the Sothern Taiwan. In 1998, A. formosana was categorized at the critically endangered level in the IUCN Red List of Threatened Species based on only population with thousands individuals. Based on 2007 species inventory data, there are 2035 individuals restricted in two mountain tops including Mt. Chachayalaisham and Jinshueiying. In this study, we estimate the genetic diversity, effective population size, and identify the distinct genetic units based on fifteen polymorphic microsatellite loci. Totally, 472 samples were survey, including 383 and 89 from Mt. Chachayalaisham and Jinshueiying, respectively. The Na, Ne, HO, HE, and PIC respectively,ranged from 2 to 10, 2.40 to 3.14, 0.52 to 0.60, 0.28 to 0.35, and 0.26 to 0.86. Most loci are significant deviations in Hardy-Weinberg equilibrium and the mean of inbreeding coefficient is displayed positive (FIS=0.48), revealing the genetic fixation causing by inbreeding, genetic drift, or both. Small effective population size (1716 and 457 individuals for subpopulation of Mt. Chachayalaisham and Jinshueiying) and large ancestral population size (232898 individuals) were estimated based on the test of isolation-with-migration model, exposing the range contraction. Bayesian clustering algorithms and Markov chain Monte Carlo (MCMC) iterations to distinct genetic units and make assignments in the programs STRUCTURE, InStruct, and GENELAND, the largest posterior probability occurred at 2 among three different analyses speculate the two subpopulations of A. formosana isolated and drift into different genetic units. In addition, the largest posterior probability occurred at 10 and 10 in Mt. Chachayalaisham and Jinshueiying based on GENELAND analysis, revealing the different distinct genetic units within subpopulations. Meanwhile, combined the location, age structure based on diameter at breast height (DBH) data, and genetic information, we establish the population structure and individual relationship of A. formosana and select individuals with different genetic units for seed source in order to maintain the maximum genetic diversity of A. formosana.

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1 - National Sun Yat-sen University, Department of Biological Sciences, No. 70, Lienhai Road, , Kaohsiung, 80424, Taiwan
2 - National Pingtung University of Science and Technology, Department of Graduate Institute of Bioresources, Pingtung, 91201, Taiwan
3 - National Pingtung University of Science and Technology, Department of Forestry, Pingtung, 91201, Taiwan

Amentotaxus formosana
assignment test

Presentation Type: Poster:Posters for Topics
Session: P
Location: Battelle South/Convention Center
Date: Monday, July 9th, 2012
Time: 5:30 PM
Number: PCB005
Abstract ID:430

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