Abstract Detail
Molecular Biology Markova, Dragomira [1], Mason-Gamer, Roberta [1]. Evolutionary Dynamics of Pong-like Transposable Elements in the wheat tribe (Triticeae). Transposable elements (TEs) are major components of all characterized eukaryotic genomes. In the wheat tribe Triticeae they account for 70-75 % of the genome. Pong-like TEs were first identified as active elements among Class 2 (DNA) transposons in the sequenced rice genome and later shown to be founding members of a new and large superfamily of transposons called PIF/IS5. Pong-like elements are autonomous elements that are about 5kb in length and contain two open reading frames (ORFs): ORF1 and ORF2. ORF2 encodes a transposase (TPase) most likely responsible for the mobilization of the element. Here we present the first comprehensive characterization and analysis of Pong-like TEs in the majority of Triticeae diploid genomes. We examine their diversity, distribution and evolution, and survey for the presence of active Pong elements. Phylogenetic analysis of 241 Pong-like TPase fragments from 22 diploid Triticeae species revealed the presence of multiple distinct lineages with two lineages significantly more diverse than the others. Our results suggest that Pong-like elements are diverse and widespread with plethora of element-associated transposases in Triticeae that originated prior to the formation of the tribe itself. Broader Impacts:
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1 - University of Illinois at Chicago, Biological Sciences, 840 West Taylor Street, Chicago, IL, 60607, USA
Keywords:
Transposable elements
Triticeae
wheat
Pong-like
phylogeny.
Presentation Type: Oral Paper:Papers for Topics
Session: 8
Location: Union B/Hyatt
Date: Monday, July 9th, 2012
Time: 10:15 AM
Number: 8001
Abstract ID:846 | 